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Table 3 Putative Dbf2-Mob1 substrates from proteome chip screen

From: Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening

ORF Name Common Name Slide Signal (after normalization)
YJR060W CBF1 118.4224
YAL051W OAF1 81.8589
YBR138C HDR1 8.9063
YOL012C HTZ1 4.0738
YMR165C SMP2 3.2182
YPL150W   3.1545
YDR226W ADK1 3.1016
YMR229C RRP5 2.6954
YBR118W TEF2 2.619
YJL108C PRM10 2.5802
YPR091C   2.5784
YKL168C KKQ8 2.5013
YNL101W AVT4 2.1486
YBR285W   1.9355
YMR239C RNT1 1.593
YNR047W   1.4015
YJL076W NET1 1.3521
YNL155W   1.2912
YNR006W VPS27 1.1429
YIL135C VHS2 1.116
YMR184W   1.1144
YDL220C CDC13 1.0274
YBR108W   1.0216
YDL019C OSH2 0.9962
YOR362C PRE10 0.9895
YNL284CA MRPL10 0.9442
YKL077W   0.8905
YDR134C   0.8135
YDL002C NHP10 0.8102
YMR072W ABF2 0.7585
YGR038CA   0.7292
YOR228C   0.6681
YKL140W TGL1 0.6321
YDL070W BDF2 0.6162
YCR105W ADH7 0.6146
YBL106C SRO77 0.5988
YNL125C ESBP6 0.5694
YHR182W   0.4773
YJL213W   0.4755
YDR299W BFR2 0.47
YPL211W NIP7 0.4521
YML037C   0.4503
YDR171W HSP42 0.442
YOL104C NDJ1 0.3611
YKL146W AVT3 0.3592
YGL245W   0.2863
YIL010W DOT5 0.2606
YNL007C SIS1 0.2239
YHL021C   0.2094
YMR196W   0.2089
YJR142W   0.2005
YGR220C MRPL9 0.1976
YLR177W   0.1626
YJL211C   0.1594
YML035C AMD1 0.1384
YGL105W ARC1 0.1332
YGR264C MES1 0.1328
YPL257WA   0.1302
YBL024W NCL1 0.1294
YJR094WA   0.1141
YLR007W NSE1 0.0988
YLR303W MET17 0.0985
YGR223C   0.0837
YKR022C   0.0806
YLL008W DRS1 0.0702
YFR033C QCR6 0.042
YLR004C   0.0228
  1. Activated FHHDbf2-H6Mob1TM9 was used to screen the yeast proteome chip. Of 86 proteins phosphorylated by FHHDbf2-H6Mob1TM9, 67 were determined to be putative substrates after taking into account proteins that were phosphorylated in the control slides treated with the kinase-inactive FHHDbf2(N305A)-H6Mob1TM9.