Figure 2From: Nonnatural amino acid incorporation into the methionine 214 position of the metzincin Pseudomonas aeruginosa alkaline proteaseSDS-PAGE gel summary of the refolding of mature full-length AprA. S represents the supernatant of the crude lysate, W1 is the solution from the first wash of the inclusion body pellet, RF is the refolded protein and HS is the refolded protein after the high salt wash and DA is the refolded DFM-AprA.Back to article page