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Table 2 Comparison of NADP+-dependent isocitrate dehydrogenases from different organisms.

From: Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation

Organism Km(isocitrate) Km(NADP+)
Blastocladiella emersonii 20 μM 10 μM
Chlorobium limicola 45 μM ± 13 27 μM ± 10
Bacillus substilis 5.9 μM ± 0.9 14.5 μM ± 2.2
Escherichia coli 4.9 μM ± 0.2 19.6 μM ± 3.6
Synechocystis sp PCc 6803 59 μM 12 μM
Pyrococcus furiosus - 4400 μM
Aeropyrum pernix - 30 μM
Thermotoga moritima - 55.2 μM
Aeropyrum fulgidus - 30 μM
Mycobacterium phlei (ATCC-354) 74 μM 53 μM
M. tb ICD-1 10 μM ± 5 125 μM ± 5
M. tb ICD-2 20 μM ± 1 19.6 μM ± 6
Beef liver NADP+-IDH 1.7 μM 7.3 μM
Rat liver (cytosolic) 9.7 μM ± 2.9 11.5 μM ± 0.2
Porcine heart NADP+ IDH - 5 μM ± 0.19