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Table 1 Gradient profile. Gradient profile used for the separation of PEth with the HPLC method.

From: Methodological modifications on quantification of phosphatidylethanol in blood from humans abusing alcohol, using high-performance liquid chromatography and evaporative light scattering detection

 

Mobile phase

Time (min)

%A

%B

%C

0–3

85

1

14

3–8

85-63

1–23

14

8–12

63-36

23–50

14

12–13

36-0

50–86

14

13–23

0

86

14

23–28

0–85

86-1

14

28–58

85

1

14

  1. A Hexane
  2. B Propan-1-ol:water; 17:3 v/v
  3. C Propan-1-ol:acetic acid:triethylamin; 316:16:1 v/v
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BMC Biochemistry

ISSN: 1471-2091

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