Figure 5From: A two disulfide bridge Kazal domain from Phytophthora exhibits stable inhibitory activity against serine proteases of the subtilisin familyCoimmunoprecipitation of the recombinant Kazal domains and P69 subtilases using FLAG antisera. Eluates from coimmunoprecipitation of rEPI1a, rEPI1b or buffter with proteins in BTH-treated tomato intercellular fluids were run on SDS-PAGE gel followed by sequential immunobloting with P69 (α-P69) and FLAG (α-FLAG) antisera at a dilution of 1:3000.Back to article page