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Figure 4 | BMC Biochemistry

Figure 4

From: A two disulfide bridge Kazal domain from Phytophthora exhibits stable inhibitory activity against serine proteases of the subtilisin family

Figure 4

The atypical Kazal domain rEPI1a inhibits P69B subtilase. A, In-gel protease assay of Nicotiana benthamiana intercellular fluids expressing the empty binary vector pCB302-3 (-) or pCB-P69B (+). B, Inhibition assay of P69B by recombinant EPI1 entire protein and the single Kazal domains. P69B-expressing N. benthamiana intercellular fluids were incubated in the presence of rEPI1, rEPI1a, rEPI1b or the absence of protease inhibitors (Buffer) and then the remaining protease activity was analyzed using zymogen in-gel protease assays. The arrow indicates the band location corresponding to the protease activity of P69B.

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