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Table 2 Generalization of discontinuous hammerhead ribozyme design

From: Low-magnesium, trans-cleavage activity by type III, tertiary stabilized hammerhead ribozymes with stem 1 discontinuities

 

[MgCl 2 ], mM

F ∞

F a

k a

k b

init rate, min -1

RzC4 [37]

5

0.32

 

0.24

 

0.077

 

2

0.27

 

0.13

 

0.036

 

0.5

0.35

 

0.055

 

0.019

RzC-ras [37]

5

0.69

 

1.21

 

0.83

 

2

0.69

0.45

1.44

0.066

0.48

 

0.5

0.71

 

0.017

 

0.012

RzD [37]

5

0.52

 

0.125

 

0.065

 

2

0.0135

 

0.0175

 

0.0024

 

0.5

0.812

 

0.00032

 

0.00022

  1. Generalization of discontinuous hammerhead design, following same conventions as in Table 1. Most data fit well to a single-exponential rate equation except for RzC-ras at 2 mM MgCl2, for which a double-exponential rate equation was used.
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BMC Biochemistry

ISSN: 1471-2091

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