Figure 2

Inhibition of lysosomal acidification by the novel inhibitors. Potoroo kidney cells (PtK₂) were treated with V-ATPase inhibitors for 4 hours and stained for lysosomes (red) with the acidotropic reagent LysoTracker, for mitochondria with MitoTracker (A, green) or for nuclei with Hoechst 33258 (B, blue). The control cells show many red vesicles indicating acidic lysosomes whereas in cells treated with the inhibitors only few red spots can be observed.