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Figure 2 | BMC Biochemistry

Figure 2

From: Characterization of 17α-hydroxysteroid dehydrogenase activity (17α-HSD) and its involvement in the biosynthesis of epitestosterone

Figure 2

Identification by HPLC of epiT produced from HEK-293 cells stably expressing 17α-HSD. (A) Elution profile of non labeled steroids; 4-dione (1st peak), testosterone (2nd peak) and epitestosterone (3rd peak). (B) Products extracted from enzymatic assay done with cells stably expressing 17α-HSD, substrate is 4-dione. (C) Products extracted from enzymatic assay done with purified enzyme; substrate is 4-dione. Separation and identification of metabolites were performed as described in Materials and Methods.

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