Figure 4From: Characterization of the aggregates formed during recombinant protein expression in bacteriaChaperone-dependent in vitro disaggregation. Purified GFP-GST aggregates recovered from the fractions 3 and 4 of the sucrose gradient were incubated in the presence of an equimolar mixture of DnaK, DnaJ, GrpE, and ClpB in the presence of a system constantly providing ATP. The aggregation index was repeatedly measured during a 45 min incubation.Back to article page