Skip to main content

Table 1 Kinetic parameters and thermal stability of PQQGDH-Bs.

From: Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface

 

V max(U/mg)

K m(mM)

Th(°C)a

t1/2 (min)b

Wild type

3030

20

53.9 ± 0.9

9.5 ± 1.4

Asn340Phe/Tyr418Phe

3100

20

57.7 ± 0.4

14.9 ± 1.1

Asn340Phe/Tyr418Ile

2500

20

57.5 ± 0.3

15.5 ± 1.5

Thr416Val/Thr417Val

2800

16

56.5 ± 0.9

14.8 ± 1.4

  1. a Th represents the temperature at which half the initial activity is lost in 10 min.
  2. b t1/2 represents the half-life at 55°C.