Table 1 half-life (t 50 ) of the wild type protein.
From: Mutation of exposed hydrophobic amino acids to arginine to increase protein stability
Denaturing agent | t50 (min) |
|---|---|
50°C | 8 +/- 1.7 |
4 M Urea | 11 +/- 1.6 |
20% acetonitrile | 1.45 +/- 0.18 |
0.1 mg/ml pronase | 14.4 +/- 2.2 |