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Table 1 half-life (t 50 ) of the wild type protein.

From: Mutation of exposed hydrophobic amino acids to arginine to increase protein stability

Denaturing agent t50 (min)
50°C 8 +/- 1.7
4 M Urea 11 +/- 1.6
20% acetonitrile 1.45 +/- 0.18
0.1 mg/ml pronase 14.4 +/- 2.2