Figure 3
From: Mutation of exposed hydrophobic amino acids to arginine to increase protein stability
Effect of replacement of hydrophobic residues at the surface by arginine on relative stability. For each mutation, ratio of t50 (t50 mutant/t50 wild type) was calculated for each denaturation agent. * indicates a significant difference with the wild type protein with P < 0.05. Mutations are arranged from the less to the more stable, considering a mean stability for the four parameters analyzed.