Figure 8

Sequence alignment of the CPSase(s) from E. coli and S. cerevisiae . The amino acids sequences of the CPSase(s) from E. coli and S. cerevisiae were aligned as indicated in the Methods. CARB-E : E. coli; PYR1-Y: S. cerevisiae. ([ ]), limits of the sequence used for the modeling process. (↑), limit between A and B domains. (◆), point mutations which abolish the sensitivity of the ATCase domain to UTP (see table 1). (*) lysine992 and serine948 residues located in the UMP binding site of the E. coli CPSase.