Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase: a two-step enzymatic procedure

BMC Biochemistry

Table 1 NodH specificity towards differently substituted LCOs.

Substrates	Incorporation of ³⁵S into LCOs^a
NodSm-IV(Ac, C16:2Δ2,9)	80 +/- 3.4
NodSm-IV(C16:2Δ2,9)	79 +/- 2.3
LCO-IV(C18:1Δ9)	77 +/- 2.5
LCO-IV(C16:1Δ9)	82 +/- 2.7
LCO-V(C16:1Δ9)	38 +/- 1.2
LCO-III(C16:1Δ9)	19 +/-0.8
LCO-II(C16:1Δ9)	6 +/-0.2
LCO-V(C16:1Δ9) reduced^b	0
NodRt-V(Me, C16:0)	38 +/- 4.3
NodRt-V(Me, C18:1Δ11)	40 +/- 2.7

^aValues are expressed as percentage of total offered ³⁵S-PAPS and represent the means +/- S.D. of 3 to 6 experiments. ^bThe anomeric carbon was reduced. The nomenclature used for LCOs and Nod factors correspond to that established in [1].

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