Skip to main content

Table 1 NodH specificity towards differently substituted LCOs.

From: Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase: a two-step enzymatic procedure

Substrates Incorporation of 35S into LCOsa
NodSm-IV(Ac, C16:2Δ2,9) 80 +/- 3.4
NodSm-IV(C16:2Δ2,9) 79 +/- 2.3
LCO-IV(C18:1Δ9) 77 +/- 2.5
LCO-IV(C16:1Δ9) 82 +/- 2.7
LCO-V(C16:1Δ9) 38 +/- 1.2
LCO-III(C16:1Δ9) 19 +/-0.8
LCO-II(C16:1Δ9) 6 +/-0.2
LCO-V(C16:1Δ9) reducedb 0
NodRt-V(Me, C16:0) 38 +/- 4.3
NodRt-V(Me, C18:1Δ11) 40 +/- 2.7
  1. aValues are expressed as percentage of total offered 35S-PAPS and represent the means +/- S.D. of 3 to 6 experiments. bThe anomeric carbon was reduced. The nomenclature used for LCOs and Nod factors correspond to that established in [1].