Figure 1From: Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase: a two-step enzymatic procedureTime course of PAPS formation. The reaction mixture containing 26 μM Na2SO4 (10 μCi of labeled sulfate) was incubatedat 37°C in 20 mM Tris-HCl buffer pH 8.5, 40 mM MgCl2, 4 mM ATP. The reaction was started by addition of 30 μl of yeast extract, either (A) without GTP (■) or (B) in the presence of 10 mM GTP (●).Back to article page