Figure 5

Tissue distribution of mouse peroxisomal 3-ketoacyl-CoA thiolase A+B activities. a) Sample immunodepletion with antibody anti-peroxisomal 3-ketoacyl-CoA thiolase A and B proteins (anti-PTL). The samples of homogenate before and after immunodepletion with the indicated amount of the PTL antibody were subjected to electrophoresis by SDS/PAGE and incubated after Western blot with the anti-PTL antibody (lane a) or with the anti-T1 antibody directed against the mitochondrial thiolase (lane b). The approximate size of the two proteins (in kDa) is indicated on the right. b) Total peroxisomal 3-ketoacyl-CoA thiolase A+B activities were measured in different tissues by subtracting activities determined on homogenate after immunodepletion from activities determined on homogenate before immunodepletion. Thiolase activities in different tissues are indicated in percentages in the bar chart and values were fixed to 100% for the liver. Means ± S.E.M of thiolase activities correspond to three independent experiments performed on three mice. No significant difference (NS) between liver and the other three tissues was found with the Student's t test (P > 0.05).