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Figure 4 | BMC Biochemistry

Figure 4

From: Molecular cloning, gene structure and expression profile of two mouse peroxisomal 3-ketoacyl-CoA thiolase genes

Figure 4

Mouse peroxisomal 3-ketoacyl-CoA thiolase A and B mRNA levels in different tissues and effect of fenofibrate on their expression in liver. a) Total RNAs (15 μg per lane from 3 mice) extracted from different tissues were hybridized with 32P-labelled cDNA probes: upper panel, mouse peroxisomal 3-ketoacyl-CoA thiolase A (mThA); middle panel, mouse peroxisomal 3-ketoacyl-CoA thiolase B (mThB); lower panel, 36B4 (acidic ribosomal phosphoprotein (P0)) as loading control. Peroxisomal 3-ketoacyl-CoA thiolase A (mThA) or thiolase B (mThB) mRNA signal was quantified, standardized with 36B4 mRNA signal for the same tissue and expressed as a percentage in comparison with the signal observed in liver for each isoform. b) Relative proportions of thiolase A and B mRNAs in mouse liver by Slot blot analysis. 1, 2, 5 and 10 μg total RNAs extracted from mouse liver were deposed onto nitrocellulose membrane and then hybridized with 32P-labelled cDNA probes: upper panel, mouse peroxisomal 3-ketoacyl-CoA thiolase A (mThA), and lower panel, mouse peroxisomal 3-ketoacyl-CoA thiolase B (mThB). The total signals of thiolase A and thiolase B were fixed to 100%. Relative proportions of thiolase A and B mRNAs were calculated and expressed as a percentage. c) Total RNAs (15 μg per lane from 3 independent animals for each condition) extracted from mouse liver of control and fenofibrate-treated mice were loaded. The membrane was hybridized with 32P-labelled cDNA probes: upper panel, mouse peroxisomal 3-ketoacyl-CoA thiolase A (mThA); middle panel, mouse peroxisomal 3-ketoacyl-CoA thiolase B (mThB); lower panel, 36B4 (acidic ribosomal phosphoprotein (P0)) as loading control. Fold variation represents mThA (or mThB) mRNA / 36B4 mRNA variation. These values were fixed to 1 for liver of untreated mice.

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