Figure 2

Nucleotide sequence and deduced aa sequence of the mThB cDNA and comparison with mThA cDNA. The complete nucleotide sequence for mThB cDNA and its deduced aa sequence are shown in full. Differences with mThA cDNA are indicated above the mThB sequence, whereas differences in the deduced aa sequence are indicated below. The positions of nucleotides and aa residues are given on the right. Nucleotide 1 (for each cDNA) corresponds to the major transcription initiation site determined by ribonuclease protection assay (figure 1c). The start codon (ATG) is indicated in bold and underlined, and the stop codon (TGA) is in bold and denoted by End. The coding regions are written in capital letters, whereas the untranslated regions are in lower case letters. Dashes (-) correspond to the absence of 5' and 3'-untranslated extension in each cDNA. The 5'-extension of mThA cDNA (124 nt) appears in italics. The underlined sequences correspond to specific probes (used for Northern blot) of thiolase A and B cDNAs, respectively. The putative polyadenylation signal AGTAAA is double-framed in the two sequences. The putative Peroxisomal Target Signal (PTS 2) is framed and shaded. The three thiolase signature patterns are framed in dotted lanes. The putative catalytic residues (Cys123, His377 and Cys408) are framed. Arrows indicate the putative cleavage site for the mature thiolase proteins. The thiolase A and B cDNAs have been deposited in the GenBank database: accession nos AY273811 and AY273812, respectively.