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Table 1 Relative activities for each protein calculated from the LPA phosphate release assay and densitometry.

From: Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity

Consruct

Mean n.Mol. PO4 released +/- SD

Mean PO4 released/unit density +/- SD

Relative activity

WunM3

1.392 +/- 0.340

0.069 +/- 0.012

1.00

WunD2M3

1.059 +/- 0.068

0.078 +/- 0.004

1.13

WunD:248>TM3

~0.000 +/- 0.026

0.000 +/- 0.001

0.00

Untransfected S2

~0.000 +/- 0.120

N/A

0.00

  1. Each immunocaptured enzyme was incubated in triplicate with LPA in the PiPer® phosphate release assay, and the nMoles phosphate released for each individual sample calculated from a standard curve. The means of the three readings for each enzyme is presented, +/- SD. Less than 3% of the total LPA was broken down in any of the experiments. The density for each individual sample on a Western blot was calculated using Bio-Rad Quantity One, and used to calculate nMoles PO4 released per unit density. The data presented is the mean of these values, +/- SD. This was then converted into relative activity.
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BMC Biochemistry

ISSN: 1471-2091

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