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Figure 2 | BMC Biochemistry

Figure 2

From: Analysis of the kinetic mechanism of recombinant human isoprenylcysteine carboxylmethyltransferase (Icmt)

Figure 2

Distinguishing ping-pong vs. sequential kinetic mechanisms for human Icmt. A. Lineweaver-Burk plot for the methylation of BFC carried out in the presence of (◆) 2 μM, (□) 4 μM and (▲) 8 μM of BFC and varied [AdoMet]. B. Lineweaver-Burk plot for the methylation of BFC carried out in the presence of (◇) 5 μM, (■) 10 μM, (○) 20 μM of AdoMet and varied [BFC]. For both experiments, the incubation time was 20 min and 0.5 μg of Sf9 membranes containing recombinant human Icmt protein were used for each condition. Data shown are the mean of duplicate determinations from a single experiment, and are representative to two such experiments.

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