Table 2 Molecular weight analysis of R. capsulatus PBGS as measured by equilibrium sedimentation. Data were collected at 4°C. The column headings refer to RPM values. All results are in Da. The monomer molecular weight is 35,857 Da.
From: Rhodobacter capsulatus porphobilinogen synthase, a high activity metal ion independent hexamer
Sample | 8000 | 10000 | 12000 | 14000 | Overall |
|---|---|---|---|---|---|
12.3 μM (.1 mM DTT) | 221,698 | 222,291 | 213,584 | 206,743 | 215,700 ± 9,700 |
12.3 μM (1 mM βME) | 227,785 | 230,906 | 224,189 | 205,239 | 220,200 ± 9,100 |