Figure 2From: Rhodobacter capsulatus porphobilinogen synthase, a high activity metal ion independent hexamerSDS-PAGE (4–20%) of PBGS Purification Steps. Lane M is marker; lane 1 is Crude Extract; lane 2 is Ultracentrifuge supernatant; lane 3 is 25% ammonium sulfate pellet; lane 4 is pooled Phenyl-Sepharose fractions; lane 5 is pooled DEAE fractions; and lane 6 is pooled S-300 fractions.Back to article page