Figure 1
From: Selective inhibition of c-Myb DNA-binding by RNA polymers
Inhibition of c-Myb DNA-binding by homoribopolymers. Panel A: NR1R2R3 (40 fmol) and R2R3 (20 fmol) in mixture were incubated with 30 fmol MRE-containing DNA probe and 1 ng (lane 4,7,10 and 13), 10 ng (lane 5, 8, 11 and 14) and 100 ng (lane 6, 9, 12 and 15) of homoribopolymers poly(A) (lane 4–6), poly(C) (lane 7–9), poly(G) (lane 10–12) and poly(U) (lane 13–15). The binding reactions were incubated for 15 minutes at 25°C and subsequently analyzed by EMSA and phosphorimaging. Lane 1, 2 and 3 show binding reactions without homoribopolymer addition, with NR1R2R3 alone in lane 1, R2R3 in lane 2 and the mixture of both in lane 3. Panel B: NR1R2R3 and R2R3 were combined in a binding reaction as in panel A, but now in the presence of 1 ng, 10 ng or 100 ng of the ribopolymers poly(G) (lanes 3–5), poly(I) (lanes 6–8), poly(I-C) (lanes 9–11) and 100 ng poly(A) (lane 12), respectively. Lane 1 shows free probe, whereas lane 2 shows binding reaction without ribopolymer addition.