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Figure 5 | BMC Biochemistry

Figure 5

From: Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase δ

Figure 5

Effect of various purified PCNA fractions on calf thymus pol δ•PCNA•32P-labeled oligonucleotide template-primer complex formation. Complex formation among pol δ, various purified PCNA fractions and 32P-labeled synthetic oligonucleotide template-primers (30-21-mers) was monitored by standard non-denaturing PAGE-band-mobility-shift assays [32]. Each incubation contained 10 ng of pol δ, 70 ng of PCNA and 0.1 pmol/reaction (useable 3'-OH) of annealed template-primer. NH2-terminally his-tagged-PCNA fractions are as indicated; wt, wild-type; Q123V, recombinant Drosophila PCNA containing a single amino acid, glutamine at position 123, changed to valine; dr119-133h, recombinant Drosophila PCNA containing the entire interdomain connector loop (amino acids 119-133) replaced with the corresponding human PCNA amino acids.

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