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Figure 3 | BMC Biochemistry

Figure 3

From: Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase δ

Figure 3

Effect of various purified PCNA fractions on the DNA polymerase activity of calf thymus pol δ. Calf thymus pol δ was incubated in a reaction mixture as described (see Materials and Methods) for 5 min at room temperature. Each incubation contained 10 ng of pol δ. DNA product synthesized was determined after placing 5-μl aliquots on Whatman DE-81 filters and subsequently washing with a 5% (w/v) solution of Na2HPO4•12H2O. Radioactivity retained on filters was then determined by liquid scintillation counter. Reaction mixtures contained increasing amounts, as indicated on the abscissa, of various PCNA samples, also as indicated.

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