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Figure 5 | BMC Biochemistry

Figure 5

From: Characterization of yeast histone H3-specific type B histone acetyltransferases identifies an ADA2-independent Gcn5p activity

Figure 5

ADA3, but not ADA2, is essential for HatB3.1 activity. Nuclear extracts from isogenic Δada2 and Δada3 strains were prepared and fractionated as previously described for wild type and HAT deletion strains (Figures 1 and 3). Fluorograms reflecting HAT activity assays from fractions of equivalent conductivity from each strain, using free histones, are shown (fraction numbers are displayed at bottom [compare lanes to those in figure 3 as well]). Regions of HatB3.1 and HatB3.2 are highlighted by brackets.

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