Figure 8

Assay of reelin within archival clinical samples. a) Graph of immunoblot band intensity vs. volume loaded for recombinant reelin (solid) and human plasma (open). b) "Raw" 310 kDa reelin band optical density scores from samples assayed pairwise (patient having only ≤10 CGG repeats vs. having at least one allele > 10 repeats; shown are the values for 0.37 μl plasma loaded per lane) as described in the text and summarized as ratios in Table I. Overall, the ''short'' repeat group had raw optical density scores of 22.3 ± 0.87 s.e.m. whereas the ''long'' repeat group had 16.9 ± 1.2. c) Assay from patient pair #4 (Table I). The patient having genotype 8/8 is on the left, and having 8/13 is on the right; each is loaded at 0.15, 0.3, 0.6 μl per lane and blotted with antibody 142. The lane at the far left is recombinant reelin standard. d) The same blot was reblotted with antibody directed against the N-terminus of amyloid precursor protein (APP), revealing a band migrating at 180 kDa.