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Figure 4 | BMC Biochemistry

Figure 4

From: Methodological factors influencing measurement and processing of plasma reelin in humans

Figure 4

Reelin is a substrate for plasminogen activator (uPA). a) Lanes 1 and 8, recombinant reelin standard. Lanes 2–4, incubated at 37 degrees for 2 10 min, 3 30 min, 4 6 h (negative controls). Lanes 5–7, incubated in the presence of uPA (3 units/ml) for 5 10 min, 6 30 min, 7 6 h. b) Lane 1, recombinant reelin standard. Lanes 2 and 3, incubated for 6 h in the presence of uPA. 2 digestion in the absence of plasminogen activator inhibitor-1 (PAI-1) and 3 digestion in the presence of PAI-1 (Calbiochem, recombinant mutant human; 0.9 mg/ml).

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