Figure 2

Excessive heating causes fragmentation of reelin. Immunoblot of recombinant reelin loaded in duplicate on a 6.5% acrylamide resolving gel with 4% stacking gel, reacted with antibody 142. Samples were prepared by adding SDS-PAGE buffer and DTT and heating at 100 C for the following times: 1 no heat, 2 1 min, 3 3 min, 4 10 min, 5 30 min. Heating resulted in progressive breakdown of reelin into a ladder of 6 additional fragments migrating between 160 and 310 kDa, plus some smaller fragments.