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Figure 7 | BMC Biochemistry

Figure 7

From: Regulated interaction between polypeptide chain elongation factor-1 complex with the 26S proteasome during Xenopus oocyte maturation

Figure 7

Changes in association between EF-1 complex and the 26S proteasome during oocyte maturation (A) Immunoblotting of the purified 26S proteasomes. 26S proteasomes were analyzed by electrophoresis under denaturing conditions (12.0% gel) and either stained with Coomassie Brilliant Blue (CBBR) or immunostained with antibodies (α-GC4/5; anti-20S proteasome α2 subunit mouse monoclonal antibody, α-EF-1γ: anti-recombinant EF-1γ) after electroblotting. Lanes I and M indicate 26S proteasomes from immature and mature oocytes, respectively. (B) Oocyte extracts from Xenopus immature and mature oocytes were immunoprecipitated using affinity-purified anti-goldfish 26S proteasome. Precipitates were immunoblotted with antibodies. Lanes I and M indicate extract from immature and mature oocytes, respectively. Protein bands of EF-1γ (p48) and α2 subunit of 20S proteasome (p25) are indicated by arrows. Molecular masses of standard proteins are indicated at the left.

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BMC Biochemistry

ISSN: 1471-2091

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