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Figure 3 | BMC Biochemistry

Figure 3

From: Regulated interaction between polypeptide chain elongation factor-1 complex with the 26S proteasome during Xenopus oocyte maturation

Figure 3

Identification of p48 complex as an EF-1 complex Purified fractions were separated by electrophoresis under denaturing conditions (12.0% gel) and stained with Coomassie Brilliant Blue (CBBR) or immunostained with antibodies (α-p48: anti-p48 fraction, α-EF-1γ: anti-recombinant EF-1γ, α-p30: anti-p30 monoclonal antibody) after electroblotting. Lanes 1 to 5 were as follows; 1, purified p48 fraction; 2, purified 26S proteasome from immature oocytes; 3, recombinant EF-1β; 4, recombinant EF-1γ; 5, recombinant EF-1δ. Protein bands are indicated by arrows. Molecular masses of standard proteins are indicated on the left.

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