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Table 4 The second-order rate constants of wtPAI-1, E351A and E351R towards tPA, uPA, plasmin and thrombin.

From: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases

 

tPA (107 M-1s-1)

uPA (107 M-1s-1)

Thrombin (103 M-1s-1)

Plasmin (104 M-1s-1)

wtPAI-1

2.11 ± 0.83

0.48 ± 0.27

0.72 ± 0.31

3.10 ± 1.15

E351A

1.25 ± 0.49

2.64 ± 0.53

1.74 ± 0.35

6.29 ± 1.83

E351R

1.52 ± 0.35

3.62 ± 0.74

3.30 ± 0.42

7.12 ± 1.96

  1. The second-order rate constants of inhibition, expressed in M-1 s-1 were determined as described in the Materials and Methods section. Values are averages of at least four independent experiments.
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BMC Biochemistry

ISSN: 1471-2091

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