Skip to main content

Table 1 Assignment of major peptide ions observed in the MALDI-ToF mass spectrum of the EndoLysC protein digest Mass values are monoisotopic for [M+H]+ ions. For the N-terminal peptide, the calculated mass takes into account a modification by methionine excision (- 131.04 Da) and acylation (+ 42.01 Da), the theoretical mass of the unmodified peptide being 1568.80 Da.

From: The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase

Measured mass (Da)

Calculated mass (Da)

Stretch location

Sequence ions identified by PSD analysis

1051.48

1051.51

17–24

 

1076.49

1076.53

282–290

 

1201.67

1201.64

273–281

 

1479.76

1479.77

2–12

y2-y4, b2-b8, a5-a8, H and Y immonium ions

1538.92

1538.94

305–317

 

1559.78

1559.80

13–24

 

1732.96

1732.98

291–304

 

1792.97

1792.98

224–240

 

1845.99

1845.98

318–334

y2-y4, b2-b5, a4-a7, R,V and Y immonium ions