Table 1 Specific and per g enzyme activities in brown trout liver
Enzymes | Activity per g of liver s-1 g-1 liver (*) or nmol min-1 g-1 liver | Specific activity s-1 mg-1 protein (*) or nmol min-1 mg-1 protein | ||||
|---|---|---|---|---|---|---|
A | B | D | E | F | ||
Peroxisomal markers | ||||||
Catalase (*) | 95.9 (0.4) | 1.3 (0.2) | 2.0 (0.3) | 3.5 (0.3) | 0.4 (0.3) | 0.6 (0.2) |
Urate oxidase | 790.4 (0.6) | 10.3 (0.6) | 13.0 (0.7) | 22.6 (0.6) | 4.7 (0.8) | 8.8 (0.8) |
D-aminoacid oxidase | 471.0 (0.6) | 6.0 (0.4) | 8.9 (0.5) | 14.4 (0.1) | 1.4 (0.4) | 3.5 (0.4) |
Fatty acyl-CoA oxidase | 73.3 (0.5) | 1.0 (0.4) | 0.4 (0.5) | 1.5 (0.3) | 0.3 (0.8) | 1.0 (0.5) |
Glycolate oxidase | 60.2 (0.5) | 0.8 (0.3) | 0.8 (0.4) | 2.3 (0.4) | 0.5 (0.5) | 0.7 (0.4) |
Mitochondrial marker | ||||||
Succinate dehydrogenase | 310.7 (0.5) | 3.6 (0.3) | 29.1 (0.5) | 27.8 (0.5) | 1.4 (0.7) | 0.2 (0.6) |
Lysosomal marker | ||||||
Aryl sulphatase | 275.0 (0.2) | 3.2 (0.4) | 10.4 (0.5) | 10.6 (0.5) | 2.4 (0.4) | 1.4 (0.4) |
Microsomal marker | ||||||
NADPH cytochrome c reductase | 2520 (0.4) | 32.5 (0.3) | 29.2 (0.4) | 41.8 (0.3) | 99.0 (0.5) | 11.5 (0.3) |
Protein per g of liver (mg g-1 liver) | Total protein per fraction (mg) | |||||
77.8 (0.2) | 225.6 (0.2) | 26.4 (0.5) | 22.3 (0.3) | 25.4 (0.6) | 128.4 (0.2) | |