Figure 5
19F NMR spectra of 6-trifluoromethyl-7-oxo-8-ribityllumazine (Compound 7). A, lumazine protein of P. leiognathi (0.13 mM protein, 0.25 mM 7); B, recombinant N-terminal domain of E. coli riboflavin synthase (0.80 mM protein, 0.98 mM 7); C, riboflavin synthase of E. coli (0.37 mM protein, 1.43 mM 7); D, riboflavin synthase of B. subtilis (0.10 mM protein, 0.75 mM 7); E, riboflavin synthase of S. pombe (0.28 mM protein, 1.22 mM 7). All samples contained 20 mM phosphate buffer, 100 mM KCl and 10% D2O, pH 7. Signals of the free ligand are indicated by a dashed line. The data were processed with a line broadening of 20 Hz.