Skip to main content
Figure 4 | BMC Biochemistry

Figure 4

From: A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically

Figure 4

The three domain form of CD147 interacts homophilically. A. BIAcore trace showing soluble rCD147d1-2rCDd3+4 at 45 μM passed over control biotinylated rCD147d1-2rCDd3+4 (1854 RU) and rCD4d1-4 (5946 RU) at 25 °C. The period of injection is indicated by the bar above the trace. There is no difference between the two flow cells showing no specific binding and the signal is due to the high concentration of protein passing over the chip. B. BIAcore trace showing four different concentrations (μM) of soluble mCD147d0-2rCDd3+4 passed over (and indicated by the bars over the trace) control biotinylated hCD5d1-3rCD4d3+4 (2033 RU) and biotinylated mCD147d0-2rCDd3+4 (1069 RU) at 37°C. C. The specific binding data in (B) i.e. after subtraction of signal over control flow cell were analysed using a non-linear curve fit graph to equation 7 [17] assuming KD1 = KD2. D. Non-linear curve fit to off rate data for soluble mCD147d0-2rCD4d3+4 dissociating at 37°C from mCD147d0-2rCD4d3+4 immobilized at 1455 RU.

Back to article page