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Figure 3 | BMC Biochemistry

Figure 3

From: A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically

Figure 3

OX114 mAb binds both two and three domain forms of mouse CD147. A. Flow cytometry of OX114 mAb binding transfected 293T cells. Light grey line represents untransfected cells, black line represents cells expressing mouse CD147d1+2, dark grey line represents cells expressing mouse CD147d0-2. B. Western blot of transfected 293T lysates. Lane U, untransfected cells, 2d, cells transfected with mouse CD147 d1+2, 3d, cells transfected with mouse CD147d0-2. Each lane contains lysate from 106 cells. C. Ability of OX114 mAb to bind to recombinant protein on the Biacore™. Proteins as illustrated in Figure 2 were immobilized on the chip, and OX114 mAb was passed over as indicated. OX114 mAb only binds to constructs containing mouse CD147d1+2.

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