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Table 1 Kinetic parameters of the mutant galactokinases. All reactions were carried out at 37°C as described in the materials and methods. Values were derived by non-linear curve fitting as implemented in the program GraphPad Prism and are shown ± standard error as derived from this procedure.

From: Sugar recognition by human galactokinase

Mutant

kcat/s-1

Km,gal/μM

Km,ATP/μM

kcat/Km,gal/l.mol-1.s-1

kcat/Km,ATP/105 × l.mol-1.s-1

Wild Type(1)

8.7 ± 0.5

970 ± 220

34 ± 4

8900 ± 2900

2.6 ± 0.4

E43A

6.7 ± 0.02

1900 ± 500

35 ± 0.3

3400 ± 430

1.9 ± 0.009

E43G

0.9 ± 0.02

140 ± 10

3.9 ± 0.6

6100 ± 330

2.4 ± 0.3

H44A

   

Not soluble

 

H44I

   

Not soluble

 

E43G/H44I

   

Not soluble

 

D46A

   

No detectable activity

 
  1. Notes (1) Previously reported in [12]