Mutant | kcat/s-1 | Km,gal/μM | Km,ATP/μM | kcat/Km,gal/l.mol-1.s-1 | kcat/Km,ATP/105 × l.mol-1.s-1 |
---|---|---|---|---|---|
Wild Type(1) | 8.7 ± 0.5 | 970 ± 220 | 34 ± 4 | 8900 ± 2900 | 2.6 ± 0.4 |
E43A | 6.7 ± 0.02 | 1900 ± 500 | 35 ± 0.3 | 3400 ± 430 | 1.9 ± 0.009 |
E43G | 0.9 ± 0.02 | 140 ± 10 | 3.9 ± 0.6 | 6100 ± 330 | 2.4 ± 0.3 |
H44A | Not soluble | ||||
H44I | Not soluble | ||||
E43G/H44I | Not soluble | ||||
D46A | No detectable activity |