Table 1 Respiratory chain enzyme activities in mycoplasma contaminated fibroblasts before and after antibiotic treatment. Values are means ± 1 SD of values obtained independently on 4 different fibroblast cultures. Data between brackets represent values for uninfected, Ciprofloxacin-naïve, fibroblasts (n > 400). NQR, NADH quinone oxidoreductase; SCCR, Succinate cytochrome c reductase; GPCCR, glycerol-3-phosphate cytochrome c reductase; QCCR, quinol cytochrome c reductase; COX, cytochrome c oxidase; CS, citrate synthase; SD, Standard deviation.
From: Mitochondrial activities in human cultured skin fibroblasts contaminated by Mycoplasma hyorhinis
Fibroblasts heavily contaminated with M. hyorhinis | Fibroblasts after Ciprofloxacin treatment | |
|---|---|---|
Enzyme activity (nmol/min/mg protein) | (n = 4) | (n = 4) |
NQR* | 20.2 ± 3.8 | 15.8 ± 0.8 |
SCCR | 22.0 ± 13.6 | 19.2 ± 3.0 |
GPCCR | 7.8 ± 3.1 | 6.7 ± 1.8 |
QCCR | 70.9 ± 25.3 | 66.6 ± 15.3 |
COX | 71.1 ± 25.3 | 49.7 ± 8.0 |
ATPase* | 31.1 ± 6.2 | 43.3 ± 7.7 |
CS | 18.8 ± 7.3 | 15.2 ± 4.2 |
Enzyme activty ratios | ||
COX/SCCR | 3.7 ± 1.14 | 2.7 ± 0.84 (3.1 ± 0.2) |
COX/GPCCR | 9.3 ± 0.84 | 8.0 ± 3.0 (7.5 ± 0.4) |
COX/QCCR | 1.0 ± 0.21 | 0.8 ± 0.29 (0.7 ± 0.05) |
QCCR/SCCR | 3.7 ± 1.15 | 3.5 ± 0.94 (4.0 ± 0.2) |
QCCR/GPCCR | 9.3 ± 1.5 | 10.5 ± 3.9 (11.7 ± 0.5) |
SCCR/GPCCR | 2.6 ± 0.61 | 2.9 ± 0.64 (2.2 ± 0.1) |
COX/CS | 4.2 ± 2.0 | 3.6 ± 1.6 (2.8 ± 0.2) |
COX/ATP* | 6.5 ± 2.0 | 2.8 ± 0.43 (3.4 ± 0.6) |
COX/NQR* | 9.9 ± 2.4 | 7.6 ± 0.49 (10.1 ± 1.6) |
ATP/NQR* | 1.6 ± 0.38 | 2.7 ± 0.61 (2.1 ± 0.4) |