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Table 2 Thermodynamic parameters (denaturant concentrations at transition midpoints and corresponding free energies extrapolated to zero denaturant concentration) for Val100Gly cpn10 denaturation by GuHCl and urea (pH 7, 20°C) at various total protein concentration.

From: Probing the interface in a human co-chaperonin heptamer: residues disrupting oligomeric unfolded state identified

Denaturant

[protein] (μM)

Midpoint (M)

ΔGU(H2O) (kJ/mol oligomer)

ΔGU(H2O) average (kJ/mol oligomer)

GuHCla

10

0.7

10 ± 1

 
 

30

0.7

14 ± 1

 
 

50

0.7

11 ± 2

12 ± 2

Ureab

30

2.25

197 ± 6

 
 

50

2.70

195 ± 6

 
 

70

3.00

200 ± 6

197 ± 6

  1. a. ΔGU(H2O) calculated using a 7-mer to 7-mer reaction mechanism. b. ΔGU(H2O) calculated using a 7-mer to 1-mer reaction mechanism.