Table 1 Thermal midpoints (Tm) observed with different Val100Gly and wild-type cpn10 total protein (monomer) concentrations (5 mM phosphate, pH 7). Error bar is ± 1°C in each case.
Protein concentration (μM) | Tm (°C) Val100Gly cpn10 | Tm (°C) wild-type cpn10 |
|---|---|---|
1 | 50.5 | 67.5 |
2 | 53 | 69.5 |
3 | 55 | 70 |
4 | - | 70.5 |
5 | 58 | - |
10 | 59 | 71.5 |
20 | 60 | - |
30 | 62 | 72.5 |
50 | 63 | 73 |