Figure 5

A. Thermal midpoints (T_m) as a function of cpn10 (total monomer) concentration (5 mM phosphate, pH 7); wild-type cpn10 (circles) and Val100Gly cpn10 (squares). Inset: Relative fluorescence (308 nm) per Val100Gly cpn10 monomer as a function of total concentration. B. DSC thermograms for 1^st (open circles; T_ms of 63°C and 72.5°C) and 2^nd (open squares; T_m of 69°C) thermal scans after mixing 40 μM Val100Gly cpn10 with 40 μM wild-type cpn10 at 20°C (5 mM phosphate, pH 7). Top: Relative fluorescence changes for 1^st (filled circles; Tms of 59°C and 71.5°C) and 2^nd (filled squares; Tm of 65°C) thermal scans after mixing 9 μM Val100Gly cpn10 with 12 μM wild-type cpn10 at 20°C (5 mM phosphate, pH 7).