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Figure 5 | BMC Biochemistry

Figure 5

From: Comparative inhibition by substrate analogues 3-methoxy- and 3-hydroxydesaminokynurenine and an improved 3 step purification of recombinant human kynureninase.

Figure 5

Inhibition of bacterial kynureninase by 3-hydroxydesaminokynurenine (4) Dixon plot illustrating inhibition of bacterial kynureninase by 3-hydroxydesaminokynurenine (4) (I = 0 (◆); I = 200 nM (▼); I = 400 nM (▲); I = 600 nM (■). The inhibition is mixed and gives a KI = 100 nM and the graph is a replot of the data (n = 3) used to construct a Michael's–Menten plot. The inset is a secondary plot of slope (from L/B) against inhibitor concentration for recombinant human kynureninase (n= 3 and r2 = 0.99) to calculate KI (5 nM). The concentration of substrate was varied between 2.5 μM – 20 μM. Lines were fitted to a straight line equation for linear regression.

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