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Table 3 Inhibition constants of ATP hydrolysis by nucleoside-3-phosphates.

From: Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase

 

GTP

CTP

UTP

dATP

dGTP

dCTP

dTTP

Ki [μM]

57 ± 20

6 ± 2

5 ± 2

3 ± 0.2

18 ± 2

9 ± 3

6 ± 2

  1. The Ki-values of inhibition of ATP hydrolysis as determined at 5 μM ATP at 70°C are summarized. 0.5 μg of purified aaTHEP1 was used for each assay in 25 μl buffer.
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BMC Biochemistry

ISSN: 1471-2091

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