Figure 7
From: Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase
aaTHEP1 hydrolyzes ATP and GTP obeying the Michaelis-Menten-equation. Steady-state kinetics of ATP (squares) and GTP hydrolysis (triangles) as catalyzed by aaTHEP1 at 70°C. Each data point for ATP hydrolysis is the mean ± SD of 4, each data point for GTP-hydrolysis the mean ± SD of 3 independent measurements. Since the signal to noise ratio of the activity measurement dramatically increases at higher substrate concentrations, 128 μM was the highest concentration under investigation. 0.5 μg of purified aaTHEP1 was used for each assay in 25 μl buffer.