Figure 2
From: Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase
Recombinant aaTHEP1 can be expressed and purified. SDS-PAGE after different steps during purification of recombinant aaTHEP1. Lane 1: crude cell extract, lane 2: supernatant after heat treatment, lane 3: eluate after cation exchange chromatography, lane 4: eluate after hydrophobic interaction chromatography. To demonstrate the purity of the final preparation, lane 4 intentionally was overloaded.