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Figure 8 | BMC Biochemistry

Figure 8

From: Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II

Figure 8

Accumulation of annexin II in the nucleus retards cell proliferation. Parental LNCaP cells and stable clones of vector-alone (GFP), GFP-WT or GFP-NES mutant annexin II were cultured as described in materials and methods for up to 8 days. Two different GFP-NES clones (NES-1 and NES-4) were included. The cell numbers on the indicated days were obtained from a standard curve (data not shown) according to the OD570–690 values. The experiment was done in triplicate and the data were analyzed using GraphPad Prism 3.0. Cell doubling time was determined for each culture, and the values were: LNCaP (30.32 ± 2.1 hours), GFP (33.8 ± 4.6 hours), GFP-WT (34.5 ± 5.1 hours) and GFP-NES (48.1 ± 8.3 hours).

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BMC Biochemistry

ISSN: 1471-2091

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