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Figure 5 | BMC Biochemistry

Figure 5

From: Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II

Figure 5

Localization of wild type and NES mutant annexin II in transiently transfected LNCaP cells. Transiently transfected LNCaP cells expressing GFP alone, GFP-wild type (GFP-WT) annexin II or GFP-NES mutant (GFP-NES) annexin II were fixed and subjected to immunocytochemistry with anti-annexin II antibody as described in materials and methods. The images were scanned using laser scanning confocal microscope (LSCM). The green color represents auto-fluorescence of GFP, the red color represents the rhodamine staining of annexin II antigen-antibody complex, and the yellow color is the overlay of GFP and annexin II images. Bar: 25 μm.

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