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Figure 4 | BMC Biochemistry

Figure 4

From: Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II

Figure 4

Northern blot analysis of annexin II in prostate cancer cells. Prior to RNA extraction, LNCaP cells of both low level and high level of confluence were treated with 10 nM dihydrotestosterone (DHT) for 2 days. 20 μg RNA were extracted from PC-3 cells (lane 1), DU-145 cells (lane 2) and LNCaP cells treated with (lanes 3, 6) or without (lanes 4, 5) DHT. [32P]-labeled annexin II cDNA was used as probe (Panel A). The location of full length annexin II cDNA is indicated by an arrow on the left side of the panel. The membrane from panel A was probed with [32P]-labeled GAPDH cDNA for normalization of the annexin II level (Panel B).

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BMC Biochemistry

ISSN: 1471-2091

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